Second level homologous MAP is indicated in cases of sterility due to alterations of the fallopian tubes, age of the female partner over 40 years, serious alterations in the quality of the seminal fluid, repeated failures of first level homologous MAP.
It consists of intense hormonal stimulation of the ovary (lasting 10-14 days), followed by transvaginal aspiration (under ultrasound control) of the oocytes contained in the ovarian follicles. The partner’s sperm are then injected into the mature oocytes thus obtained; these are then placed in an incubator where they transform into embryos (after 2-3 days) and then into blastocysts (more mature embryos that form after 5-6 days). The embryos or blastocysts are then transferred to the uterine cavity, where they have the opportunity to implant and give rise to pregnancy. Unused embryos are frozen and stored. Embryo transfer (embryo transfer) can be performed in the same stimulation cycle or in a subsequent cycle, depending on the patient’s condition.